A couple of months ago, I ran out of Invitrogen’s 5x Second Strand Buffer. I really wanted to complete cDNA synthesis on that day. Also, that buffer is unbelievably expensive for a buffer ($133/0.5 mL!). So, I looked for a recipe in my bible (Current Protocol in Molecular Biology) and yes, I found it. Here itContinue reading “5x Second Strand Buffer”
The Ikegami laboratory @ Cincinnati Children's 